Dr.Ayat

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Introduction: Candida or a yeast overgrowth may also possibly cause or aggravate a pre-existing acne condition. Candida may occur after long-term antibiotic use, which is particularly plausible when consider the nature of conventional acne treatments such as internally taken antibiotic medication and externally applied antibiotic ointments or creams. The aim of this study is to identify Candida parapsilosis from patients with acne and determine was their drugs susceptibility. Methods and Material: In this cross-sectional study of 70 clinical specimens from suspected skin with acne protests were collected by sterile swab and were streaked on SDA medium containing chloramphenicol. The plates were incubated for 48 hours in c˚37. Suspected colonies were studied through microscopic examination and subsequent passage in accordance with Mycology  of standard procedures and specify the type of fungal colony color in CHROM agar for the isolation of the yeast. For final approval, Candida Species Sequencing Method(ITS2 , ITS1 regions) was performed, and susceptibility testing was performed to review Candida for drug-resistant isolates based on CLSI method. Findings: Overal ,11 Candida species including C.parapsilosis 8 (72.73%), C.krusei 1(12.5%), C.lusitaniae 1(12.5%), C. kefyr1(12.5%), and ###i out of  the collected clinical isolates were identified and isolated. C. parapsilosis isolates susceptibility to diverse concentrations of the anti-fungal agents to isolate Cp1 study indicates that the isolated Cp8 and Cp5 with MIC 50 equal to32,0.5,0.25 and MIC90 of <64, <1, <0.5 μg/ml Fluconazole, Itraconazole and Ketoconazole were resistant respectively. Some of the isolates  having relative strength, almost all other species of C. parapsilosis isolates were susceptible to these drugs. Discussion& Conclusion: Etiological factors, pathogenesis, drug resistance and risk factors of acne and the role of yeast to induce skin disease as a contributory factor in causing acne can be a topic of interest in dermatology.Key-words: ###a href="https://www.nlm.nih.gov/cgi/mesh/2016/MB_cgi?term=Acne%20Vulgaris" target="MeSH_Browser" title="MeSH Browser: Acne Vulgaris">Acne , Candida, parapsilosis ,Antifungal Susceptibilities.